| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gustafson, Claire R (8)
|
VASRVA |
20.63 |
20.91 |
0.28 |
| 2 |
Basmajian, Kensington A (8)
|
VASRVA |
22.40 |
21.25 |
-1.15 |
| 3 |
Djordjevic, Natalija (8)
|
VASRVA |
31.54 |
21.74 |
-9.80 |
| 4 |
Shoulders, Carrington E (8)
|
VAQSTS |
22.68 |
21.88 |
-0.80 |
| 5 |
Lyons, Katharine K (8)
|
VASRVA |
21.65 |
22.07 |
0.42 |
| 6 |
Tobin, Elise M (8)
|
NCEAC |
22.57 |
22.08 |
-0.49 |
| 7 |
Hynst, Lexi M (8)
|
VASRVA |
|
23.12 |
- |
| 8 |
Lee, Hope A (8)
|
VA757 |
22.21 |
23.33 |
1.12 |
| 9 |
Ortiz, Acelynn A (8)
|
VAQSTS |
24.51 |
24.00 |
-0.51 |
| 10 |
Atkinson, Vivianne H (8)
|
VASRVA |
25.35 |
24.57 |
-0.78 |
| 11 |
Smith, Sloane A (7)
|
VAQSTS |
|
26.16 |
- |
| 12 |
Halligan, Molly Q (8)
|
VASRVA |
27.72 |
26.31 |
-1.41 |